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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 16 Documents
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Search results for , issue " Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA" : 16 Documents clear
Uji Toleransi Tanaman Kentang Hitam (Plectranthus rotundifolius (Poir.) Spreng.) Hasil Radiasi Sinar Gamma terhadap Cekaman Kekeringan Ridwan, Ridwan; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2310

Abstract

Plectranthus rotundifolius (Poir.) Spreng. is one of the potential root crops to become an alternative food. The aim of thisstudy was to determine drought resistant status of Plectranthus rotundifolius irradiated by gamma irradiation. Thisexperiment was conducted in the Green House using a Completely Randomized Design with 2 factors and 5 replications.The first factor was plant number consisted of 7 accessions (D116, D69, M343, D40, M95, D3, and Klefa Imut/KI). Thesecond factor was the level of field capacity (FC) consisted of 100% FC, 60% FC, and 20% FC. The observed parameterwere vegetative and generative growth stage, stomatal conductance, leaf water potential, and chlorophyll content. The resultshowed that the resistance level of the 7 accessions can be devided into 3 categories: 1) The plants that were susceptible todrought i.e. D116, D69, M343, and KI; 2) The plants that were semi-tolerant to drought i.e. D40 and M95; and 3) The plantthat was tolerant to drought i.e. D3.Keywords: Plectranthus rotundifolius, gamma irradiation, drought stress
Kajian gen Amely Gajah Sumatra (Elephas maximus sumatranus) Zein, Moch Syamsul Arifin; Sulandari, Sri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2319

Abstract

Sumatran elephants (Elephas maximus sumatranus) is endemic of Asian elephants in Indonesia, an endangered animal andlisted on Appendix I of CITES. Therefore, information on the status and distribution of the population of Sumatranelephants, including the distribution of genetic diversity is necessary to facilitate the development of adequate conservationand management strategies. The purpose of this research was to use Amely gene to trace paternal lineage of Sumatranelephants based on Y chromosome variation. A total of 22 blood samples of male Sumatran elephants were collected inSumatra (Way Kambas , Seblat, Bentayan, Sugihan, dan Bukit Serelo Lahat). We amplified intronic regions of the Ylinkedgene (Amely) using published primer sequences (Amely-R2 and Amely-F2) and sequenced. Sequences generatedfrom this study, aligned with reference sequences available in the GenBank, namely Elephas maximus (AY823325.1),Loxodonta Africana (AY 823320.1; AY 823321.1), Loxodonta cyclotis (AY823322.1; AY8233223.1, AY 823324.1).Neighbour Joining tree of Sumatran elephants was performed using MEGA version 5.2.2. The analysis results of 22-maleSumatran elephants, indicating that no diversity (no variation) of the Y chromosome obtained among the population ofSumatran elephants. Sumatran and Asian elephants have the same haplotypes. Further results confirmed that the savannaelephants (Loxodonta africana ) and the forest elephants (Loxodonta cyclotis ) formed two (2) separate clades, which showstwo different species. Results obtained in this study may help to design future conservation programs for the species.Keywords: Sumatran elephant, Amely gene, Y chromosome, Genbank, haplotype
Pembentukan dan Penampilan Pisang Rejang Hibrid Triploid Hasil Persilangan Pisang Rejang Mixoploid Dengan Pisang Rejang Diploid Poerba, Yuyu S.; Witjaksono, Witjaksono; Handayani, Tri
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2308

Abstract

Induction of tetraploid from diploid bananas often producing mixoploid plants.Characters of the mixoploidplant is similar to those of tetraploid plants. In this research mixoploid banana cultivar Rejang was crossed withdiploid Rejang to produce triploid hybrid Rejang. Triploid banana is one of plant breeding target because of itsbest vigor and productivity compared to diploid or tetraploid banana. 570 crosses were conducted withmixoploid Rejang as female parents and diploid Rejang as male parents. Out of 570 crosses, 130 hybrid seedswere produced. However, only 19 seeds (14.61%) contained embryos, and only two embryos were developedinto shoots. The two genotypes of developed embryos were then in-vitro propagated and planted in the field.Results of ploidy identification using Flowcytometer showed that the two genotypes were diploids (31%) andtriploids (69%). The triploid hybrid Rejang had plant growth habit of drooping, few suckers, higher and biggerpseudostem, higher bunch weight and fruit weight compared to those of the two parents. The triploid hybridhad similar genetic properties with genetic identity of 0.9174-0.9703.Keywords: Banana, crosses, diploid, Rejang, mixoploid, triploid, hybrids
Struktur dan Komposisi Jenis-Jenis Pohon di Taman Nasional Gunung Rinjani bagian Selatan, Lombok, Nusa Tenggara Barat Mansur, Muhammad
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2320

Abstract

Plant ecology study has been conducted in the southern part of Rinjani Mountain National Park on April 2015 to determinethe structure and composition of tree species. Four plots, 30 x 30 m each were established at four locations, those are; Pal-Belanda (1046 m asl), Pengorot-1 (929 m asl), Pengorot-2 (820 m asl), and Sebau (1134 m asl). The results showed thatthere are two types of forest in the sites, namely the secondary forest (Pal-Belanda, Pengorot-1 and Pengorot-2) and primaryforest (Sebau). From all plots (0.36 ha) we found 36 species of trees (trunk diameter > 5 cm) of 449 individuals, 28 genusand 21 families with a total Basal Area of 16.73 m2 (46.47 m2/ha). Shannon Diversity Index (H) in the Pengorot-2 recorded1,15, followed Pengorot-1 (1.13), Pal-Belanda (1.03) and Sebau (0.72). Saurauia pendula recorded as the dominant speciesin all site with Important Value Species (IVS) of 245% followed Dendrocnide microstigma (IVS = 162%), Platea excelsa(IVS=148.2%), Syzygium glomeruliferum (IVS=72%) and Symplocos cochinchinensis (IVS=49.3%). Myrtaceae andEuphorbiaceae were the common families in the sites. Structure and composition of tree species in the Pal-Belanda,Pengorot-1 and Pengorot-2 were relative similar (Similarity Index 70.3% and 69.4%) but different than in Sebau area(Similarity Index 24.9%).Keywords: Structure, composition, tree diversity, Rinjani, Lombok
Pengaruh Estradiol-17? dan Kolagen Tipe IV Terhadap Ekspresi Gen PIK3CA untuk Menginduksi Ekspresi c-erbB2 pada Lini Sel Kanker Ovarium SKOV-3 Martgrita, Merry Meryam; Tan, Marselina Irasonia
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2312

Abstract

Ovarian cancer cell metastasis is induced by signaling pathway activated by the binding of type IV collagen to ?1 integrinreceptor on the surface of cancer cells and estrogen binding to estrogen receptor. However, the role of estradiol-17? andtype IV collagen on the development of ovarian cancer have not been clearly understood. Therefore, this research wasconducted to observe the differential gene expression in SKOV-3 ovarian cancer cells cultured on type IV collagen andtreated with estradiol-17?, and incubated for 12, 24 and 72 hours. Differential display RT-PCR was used to express thedifferential expression gene after treatment. cDNA fragment that expressed differentially was isolated and sequenced.Sequencing result on one of the cDNA fragment showed that PI3K is one of the gene expressed in SKOV-3 ovarian cancercell. To verify this result, cDNA was amplified using PIK3CA specific primer. The increasing level of PIK3CA is inducedby three kinds of receptor activities, those are c-erbB2 receptor bound to estradiol-17?, homodimer receptor of c-erbB2, andthe activity of integrin receptor bound to type IV collagen. The increasing level and activity of PIK3CA can also increasethe expression of c-erbB2 gene. In SKOV-3 cells cultured on type IV collagen for 72 hours, the increasing of PIK3CA andc-erbB2 expression level is very low. The conclusion is that estradiol-17? gives a more significant effect than type IVcollagen to induce the increasing expression of PIK3CA and c-erbB2 genes.Keywords: ovarian cancer, SKOV-3, type IV collagen, estradiol-17?, PIK3CA, c-erbB2
Pola Distribusi dan Dinamika Komunitas Burung Di Kawasan Cibinong Science Center Widodo, Wahyu; Sulystiadi, Eko
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2326

Abstract

"Cibinong Science Center LIPI or CSC’s LIPI as an artificial habitat to the bird species conservation, especiallywith their ecological parks. This research was to study the distribution patterns and dynamics of birdcommunities in the CSC’s LIPI areas during 2005 to 2009 and on 2014. Transect Line’s method was used inthis study. The results were 39 species of birds to live in the eight sites observation on 0.27 km2 areas.However, there are no significant effects, especially relationships between Margalef’s index value to area,cotton area closest distance to the river and their habitat situation. According to “multiple linear regression”analysis show that ?= 3.626-0.69 area -1.31 cotton area closest distance to the river + 0.94 habitat situation,with determinant coefficient value (R2) = 0.161. The densities of nine species were found high. Those specieswere Lonchura punctulata (D=49.37 individuals/ha), Zosterops palpebrosus (D=22.2 individuals/ha),Collocalia linchi (D=15.00 individuals/ha), Orthotomus sutorius (D=12.70 individuals/ha), Lonchura maja(D=12.48 individuals/ha), Passer montanus (D=12.19 individuals/ha), Pycnonotus aurigaster (D=7.37individuals/ha), Dicaeum trochileum (D=6.07 individuals/ha) and Streptopelia chinensis (D=5.85 individuals/ha). The diversity indices value (H’) 1.52 to 2.51 and the evenness indices value (E) 0.52 to 0.86. Thesimilarities indices value (SI) of birds in the CSC’s LIPI will be compare to another places, i.e. the IPBcampus at Darmaga, Bogor or Yogyakarta State University’s campus in Yogyakarta showed no different,relatively. The IS value were 58.82 to 61.54%. Birds in the CSC parks were high dominant to occure in thelower strata (27.78–43.18%) and upper strata (50.43-70.83%), while in the middle strata (1.39-9.15%) tend tobe slightly. Most activities were carried by birds , especially foraging and calls. Although, the birds in the CSCparks also breeding, take a rest etc . The CSC’s LIPI parks at Cibinong could be used as a reference to thebird’s artificial habitat model to others areas. That’s an important if the areas are industries complex or theCenter office of some government buildings. But, the composition and structure of the vegetation will bedeveloped in there should be to produce some natural food resources and safety well for breeding of birds.Keywords: bird species, ecological parks, artificial habitat, distribution patterns, dynamics communities
Keanekaragaman Jenis dan Pemanfaatan Sumberdaya oleh Kelelawar Sub Ordo Megachiroptera di Taman Wisata Alam Gunung Meja Manokwari, Papua Barat Bumbut, Petrus Izak; Kartono, Agus Priyono; Maryanto, Ibnu
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1373.118 KB) | DOI: 10.14203/jbi.v12i1.2321

Abstract

Bats utilizing resources together will differ morphologically, ecologically, and behaviorally. This study aimed to measurespecies diversity and to investigate resource use by Megachiropteran bats. Data were analyzed using Principal ComponentAnalysis (PCA), Canonical Correspondence Analysis (CCA), Correspondence Analysis (CA), euclidean distance, chisquare, Anova, and niche overlap index. The result showed seven bat species were captured in three habitat types. Plant asfood identified from pollen comprised 55 species from 29 families and 11 types of corolla. Dobsonia magna and Rousettusamplexicaudatus preferred mixed fruit plantation, whilst other bat species tended to choose primary forest. Dobsonia minortended to have high niche overlap against Macroglossus minimus and Nyctemene aello. According to similarity of foodresource niche, Dobsonia magna, Dobsonia minor, and Macroglossus minimus tended to closely associate, in contrast,Rousettus amplexicaudatus, Nyctemene albiventer, Nyctemene aello, and Syconicteris australis were groupedcorrespondingly. Niche overlap index of food resource among bat species were overall less than 50%. This indicated thatthere was no interspecies competition to food resource. Coronoid process height (CPH), maximum zygomatic breadth(MZB), and condyle to canine bite point (CC) were craniodental morphology variables which did not describe certaincorolla types.Keywords: species diversity, resource use, niche overlap, Megachiropteran, Gunung Meja
Pseudozyma aphidis as inoculant for local chicken Rahmansyah, Maman; Sugiharto, Arwan; Kanti, Atit
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.679 KB) | DOI: 10.14203/jbi.v12i1.2327

Abstract

SHORT COMMUNICATION
Analisis Sekuen DNA Mitokondria Cytochrome Oxidase I (COI) mtDNA Pada Kukang Indonesia (Nycticebus spp) sebagai Penanda Guna Pengembangan Identifikasi Spesies Wirdateti, Wirdateti; Indriana, Eka; Handayani, Handayani
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1180.665 KB) | DOI: 10.14203/jbi.v12i1.2322

Abstract

Slow lories is small size primates of the genus Nycticebus that are in endangered status. Habitat pressure andhigh trade as pet animals has made the population decline both quantitatively and qualitatively, therefore it isnecessary to save the slow lories through conservation action. Morphologically, the individuals are difficult tobe distinguished among the species, therefore making much harder in identification for conservation purposessuch as from confiscated individuals. Therefore molecular marker is needed through mitochondrial DNA usingCOI gene sequences analysis is needed for identification. This study used 24 samples of slow lories consistedof 2 from Kalimantan, 9 from Java and 13 from Sumatra. The result indicated that in the 660 bp there were 62variable sites with 15 haplotypes: 4 haplotypes of N. javanicus, 9 haplotypes N. coucang, and 2 haplotypes N.menagensis. Base on nucleotide variations at specific sites, it was formed haplotype differences among species.The average genetic distance between species showed that Kalimanatan population were closer to thepopulation of Sumatra (d= 0.042 ± 0.006) compared with Java population (d= 0.059 ± 0.009), whereas thegenetic distance within population showed the population of Java had low diversity (d= 0.002) with a meannucleotide differences 2.6. Thus Jawan slow loris (N. javanicus) should be conserved.Keywords: Nycticebus spp., molecular, COI, conservation, marker
Bioacustics of Hylarana Celebensis (Peters, 1872) (Anura: Ranidae)From Sulawesi Kurniati, Hellen
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2306

Abstract

Hylarana celebensis (Peters, 1872) is an endemic frog to Sulawesi, the species being a member of family Ranidae.  The presence of the frog in its habitat is easily detected from its advertisement call; males usually call in a chorus, they call to each other in a large group. Bioacoustics of calls that a typical individual male of H. celebensis has not been described in detail, although it is very easy to find this species in freshwater swamps, permanent ponds, or slow-flowing waters in the lowland areas. The purpose of the bioacoustic analysis on H. celebensis’s calls that were recorded at Bahodopi area is to build a reference collection to be compared with H. celebensis’s bioacoustics to the other regions in Sulawesi. Because of the wide distribution of this frog in Sulawesi; population genetic structure of H. celebensis may also follow the population genetic structure of Ingerophrynus celebensis. Calls of H. celebensis have two types of calls, i.e. pure tone and pulse; however, pure tones have three variation, namely pure tone type 1, pure tone type 2 and pure tone type 3; however pulsed call has only one type. Keywords: Anura, Hylarana celebensis, bioacoustics, Sulawesi. 

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